| ชื่อโครงการวิจัย/ชื่อเรื่อง |
Development of Recombinant Probiotic for Disease Control in Nile Tilapia (Oreochromis Niloticus, Linn.). |
| ชื่อนักวิจัย/ชื่อผู้แต่ง |
Chamaiporn Chalongkhwan |
| เจ้าของผลงานร่วม |
Nontawith Areechon ,
Sasimanas Unajak |
| คำสำคัญ |
Streptococcus agalactiae;tilapia;DNA vaccine;Bacillus subtilis |
| หน่วยงาน |
Kasetsart University |
| ปีที่เผยแพร่ |
2561 |
| คำอธิบาย |
Streptococcus agalactiae is one of seriously infectious bacteria of the streptococcosis disease which causes the devastation in tilapia cultures. In this study, the development of probiotic-Bacillus subtilis as an orally DNA vaccine delivery system harboring S. agalactiae immunogenic gene was performed. The immunoreactive proteins of S. agalactiae serotype Ia and III were identified. There are 44 immunoreactive proteins identified from serotype Ia and 64 immunoreactive proteins from serotype III were isolated. Among them, it is revealed that 10 immunoreactive proteins were found in both serotype. Then, five candidate immunoreactive genes were cloned into expression vector of B. subtilis. The recombinant expression plasmids were completely transformed into B. subtilis and successfully developed recombinant B. subtilis to use as a delivery vehicle of oral DNA vaccines. Whereas, protein expression in recombinant Bacillus was found false positive results makes it needed more investigation. |
| สาขาการวิจัย |
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